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KMID : 0043320140370070890
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Archives of Pharmacal Research
2014 Volume.37 No. 7 p.890 ~ p.898
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Simultaneous analysis and peroxynitrite-scavenging activity of galloylated flavonoid glycosides and ellagic acid in Euphorbia supina
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Nugroho Agung
Rhim Tae-Jin
Choi Moo-Young
Choi Jae-Sue
Kim Youn-Chul
Kim Min-Sun
Park Hee-Juhn
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Abstract
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The herbs of Euphorbia supina (Euphorbiaceae) have been used to treat hemorrhage, chronic bronchitis, hepatitis, jaundice, diarrhea, gastritis, and hemorrhoids as a medicinal herb. This work is aimed to qualitatively and quantitatively analyze the polyphenols with peroxynitrite-scavenging activities. The eight compounds: gallic acid, methyl gallate, avicularin, astragalin, juglanin, isoquercitrin 6″-gallate, astragalin 6″-gallate, and ellagic acid, were isolated from E. supina and used for HPLC analysis and peroxynitrite (ONOO?)-scavenging assay. Simultaneous analysis of the eight compounds was performed on MeOH extract and its fractions. The contents in MeOH extract and peroxynitrite-scavenging activities of the dimer of gallic acid, ellagic acid (15.64 mg/g; IC50 0.89 μM), and two galloylated flavonoid glycosides, astragalin 6″-gallate (13.72 mg/g; IC50 1.43 μM) and isoquercitrin 6″-gallate (16.99 mg/g; IC50 1.75 μM), were high, compared to other compounds. The legendary uses of E. supina could be attributed to the high content of polyphenols, particularly ellagic acid, isoquercitrin 6″-gallate, and astragalin 6″-gallate as active principles.
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KEYWORD
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Euphorbiaceae, Euphorbia supina, Galloylated flavonoid glycosides, Peroxynitrite, HPLC
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